(d.) 20 grm. seed (sample I), previously extracted with naphtha, was extracted with chloroform, which removed a further quantity of green oil. After driving off the chloroform the oily residue was extracted with 100 c.c. water, filtered, and concentrated to 10 c.c. Of this 5 c.c. given hypodermically produced no symptoms. The conclusion to be drawn from these experiments is that the oil, or oils, has no toxic action. It is probable that the chloroform extracts contained some tutin, for tutin is soluble therein to a small extent, but the amount was either originally too small to produce symptoms or it underwent destruction in making the hot-water extracts. Methods of Extracting Tutin. Although at present there seems little likelihood that the pure substance, tutin, will ever be of any therapeutic or other commercial value, it may be of use to workers on the subject to add a note on the methods of extracting it. The best source of tutin is the seed—ripe or unripe. Drying in the air probably does not lead to any loss, but crushing and grinding, especially when combined with watery extraction and evaporation, lead to considerable loss. So far as my present experience goes, the best method is to extract the oils from the dried and recently crushed seed with mineral naphtha, and then extract the residue with ether. The ether-soluble material can then be again extracted with naphtha to remove more of the oils, and the result is an extremely toxic material, which can be further purified as described by Easterfield and Aston. Summary and Conclusions. 1. No evidence was obtained of any toxic substance in the juice of the ripe tutu-fruit. 2. The green petals of the unripe fruit contain as much tutin as other green parts of the plant. 3. Both ripe and unripe seeds contain between 0.1 per cent. and 0.6 per cent. of tutin, being about double what is found in young shoots in the natural state. (When the water percentage is taken into account there is not much difference.) 4. The constituents of the seeds soluble in naphtha and chloroform (oils and resins) were not found to possess any toxic action. 5. The sugars present in the juice of the berry appear to be a mixture of dextrose and laevulose. References. Fitchett, F., 1909. Physiological Action of Tutin, Trans. N.Z. Inst., vol. 41, pp. 286–366. (This paper gives a full list of previous work on tutin.) Malcolm, J., 1914. Some Experiments on Tutin and Tutu Poisoning, Trans. N.Z. Inst, vol. 46, pp. 248–54.